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After Miller's death in 2007, scientists examining sealed vials preserved from the original experiments were able to show that there were actually well over 20 different amino acids produced in Miller's original experiments. That is considerably more than what Miller originally reported, and more than the 20 that naturally occur in the genetic code.
After Miller's death in 2007, scientists examining sealed vials preserved from the original experiments were able to show that there were actually well over 20 different amino acids produced in Miller's original experiments. That is considerably more than what Miller originally reported, and more than the 20 that naturally occur in the genetic code.
2007年Miller去世后，科学家们检查了从原始实验中保存下来的密封小瓶，发现在米勒原始实验中事实上产生了超过20种不同的<font color="#ff8000"> 氨基酸 amino acids </font>。这大大超过了Miller最初报道的数量，也超过了<font color="#ff8000"> 遗传密码 genetic code</font>中自然产生的20种。
The original experiment remained in 2017 under the care of Miller and Urey's former student Jeffrey Bada, a professor at the UCSD, Scripps Institution of Oceanography. The apparatus used to conduct the experiment was on display at the Denver Museum of Nature and Science.
The original experiment remained in 2017 under the care of Miller and Urey's former student Jeffrey Bada, a professor at the UCSD, Scripps Institution of Oceanography. The apparatus used to conduct the experiment was on display at the Denver Museum of Nature and Science.
最初的实验在2017年由Miller和Urey以前的学生，加州大学圣地亚哥分校斯克里普斯海洋研究所教授杰弗里·巴达 Jeffrey Bada 负责<ref>{{cite news |url=https://www.nytimes.com/2010/05/18/science/18conv.html |title=A Conversation With Jeffrey L. Bada: A Marine Chemist Studies How Life Began |newspaper=nytimes.com |date=2010-05-17 |first=Claudia |last=Dreifus |authorlink=Claudia Dreifus |url-status=live |archiveurl=https://web.archive.org/web/20170118034218/http://www.nytimes.com/2010/05/18/science/18conv.html |archivedate=2017-01-18 }}</ref> {{asof|2013}}。那些实验仪器在丹佛自然科学博物馆存放展出<ref>{{cite news|url=http://www.dmns.org/science/museum-scientists/david-grinspoon/funky-science-wonder-lab/research-updates/astrobiology-collection-miller-urey-apparatus | title=Astrobiology Collection: Miller-Urey Apparatus |archiveurl=https://web.archive.org/web/20130524090309/http://www.dmns.org/science/museum-scientists/david-grinspoon/funky-science-wonder-lab/research-updates/astrobiology-collection-miller-urey-apparatus/ |archivedate=2013-05-24 |publisher=Denver Museum of Nature & Science }}</ref>{{update after|2020|4|14}}。
One-step reactions among the mixture components can produce [[hydrogen cyanide]] (HCN), [[formaldehyde]] (CH<sub>2</sub>O),<ref>https://www.webcitation.org/query?url=http://www.geocities.com/capecanaveral/lab/2948/orgel.html&date=2009-10-25+16:53:26 Origin of Life on Earth by Leslie E. Orgel</ref><ref>{{Cite book |url=http://books.nap.edu/openbook.php?record_id=11860&page=85 |title=Read "Exploring Organic Environments in the Solar System" at NAP.edu |accessdate=2008-10-25 |url-status=live |archiveurl=https://web.archive.org/web/20090621053626/http://books.nap.edu/openbook.php?record_id=11860&page=85 |archivedate=2009-06-21 |doi=10.17226/11860 |year=2007 |isbn=978-0-309-10235-3 |last1=Council |first1=National Research |last2=Studies |first2=Division on Earth Life |last3=Technology |first3=Board on Chemical Sciences and |last4=Sciences |first4=Division on Engineering Physical |last5=Board |first5=Space Studies |last6=System |first6=Task Group on Organic Environments in the Solar }} Exploring Organic Environments in the Solar System (2007)</ref> and other active intermediate compounds ([[acetylene]], [[cyanoacetylene]], etc.):{{Citation needed|date=June 2016}}
One-step reactions among the mixture components can produce [[hydrogen cyanide]] (HCN), [[formaldehyde]] (CH<sub>2</sub>O),<ref>https://www.webcitation.org/query?url=http://www.geocities.com/capecanaveral/lab/2948/orgel.html&date=2009-10-25+16:53:26 Origin of Life on Earth by Leslie E. Orgel</ref><ref>{{Cite book |url=http://books.nap.edu/openbook.php?record_id=11860&page=85 |title=Read "Exploring Organic Environments in the Solar System" at NAP.edu |accessdate=2008-10-25 |url-status=live |archiveurl=https://web.archive.org/web/20090621053626/http://books.nap.edu/openbook.php?record_id=11860&page=85 |archivedate=2009-06-21 |doi=10.17226/11860 |year=2007 |isbn=978-0-309-10235-3 |last1=Council |first1=National Research |last2=Studies |first2=Division on Earth Life |last3=Technology |first3=Board on Chemical Sciences and |last4=Sciences |first4=Division on Engineering Physical |last5=Board |first5=Space Studies |last6=System |first6=Task Group on Organic Environments in the Solar }} Exploring Organic Environments in the Solar System (2007)</ref> and other active intermediate compounds ([[acetylene]], [[cyanoacetylene]], etc.):{{Citation needed|date=June 2016}}
此外，水和甲醛可以反应，通过<font color="#ff8000"> 布特列罗夫反应 Butlerov’s reaction</font>产生各种糖，如<font color="#ff8000">核糖 ribose </font>。
此外，水和甲醛可以反应，通过<font color="#ff8000"> 布特列罗夫反应 Butlerov’s reaction</font>产生各种糖，如<font color="#ff8000">核糖 ribose </font>。
The experiments showed that simple organic compounds of building blocks of proteins and other macromolecules can be formed from gases with the addition of energy.
The experiments showed that simple organic compounds of building blocks of proteins and other macromolecules can be formed from gases with the addition of energy.
The experiments showed that simple organic compounds of building blocks of proteins and other macromolecules can be formed from gases with the addition of energy.
The experiments showed that simple organic compounds of building blocks of proteins and other macromolecules can be formed from gases with the addition of energy.
实验表明，在添加能量的情况下可以生成构成<font color="#ff8000"> 蛋白质 proteins</font>和其他<font color="#ff8000"> 大分子 macromolecules</font>的简单有机化合物 。
==Other experiments其他实验==
==Other experiments其他实验==
There also had been similar electric discharge experiments related to the origin of life contemporaneous with Miller–Urey. An article in The New York Times (March 8, 1953:E9), titled "Looking Back Two Billion Years" describes the work of Wollman (William) M. MacNevin at The Ohio State University, before the Miller Science paper was published in May 1953. MacNevin was passing 100,000 volt sparks through methane and water vapor and produced "resinous solids" that were "too complex for analysis." The article describes other early earth experiments being done by MacNevin. It is not clear if he ever published any of these results in the primary scientific literature.<!--is it not clear because academics have researched this and somehow can't tell, or is it just not clear to the Wikipedia contributor from reading only the NYT article?-->
There also had been similar electric discharge experiments related to the origin of life contemporaneous with Miller–Urey. An article in The New York Times (March 8, 1953:E9), titled "Looking Back Two Billion Years" describes the work of Wollman (William) M. MacNevin at The Ohio State University, before the Miller Science paper was published in May 1953. MacNevin was passing 100,000 volt sparks through methane and water vapor and produced "resinous solids" that were "too complex for analysis." The article describes other early earth experiments being done by MacNevin. It is not clear if he ever published any of these results in the primary scientific literature.<!--is it not clear because academics have researched this and somehow can't tell, or is it just not clear to the Wikipedia contributor from reading only the NYT article?-->
在Miller-Urey的同时期也有过类似的与生命起源有关的放电实验。《纽约时报》（1953年3月8日：E9）上的一篇题为“回首20亿年”的文章描述了在1953年5月Miller发表论文之前的俄亥俄州立大学沃尔曼.M.麦克尼文 Wollman M.MacNevin的工作。MacNevin对甲烷和水蒸气施加10万伏特的火花，产生了“树脂固体”。而这些“树脂固体”过于复杂，无法分析。这篇文章还记录了MacNevin研究早期地球的其他实验。目前还不清楚他是否曾在初级科学文献中发表过这些结果<ref>{{cite book | title=History of Shock Waves, Explosions and Impact: A Chronological and Biographical Reference | publisher=[[Springer-Verlag]] | author=Krehl, Peter O. K. | year=2009 | pages=603}}</ref>。（不清楚是因为学者们已经对此进行了研究仍不知如何判断，还是因为维基百科的撰稿人只阅读了《纽约时报》？）
K. A. Wilde submitted a paper to Science on December 15, 1952, before Miller submitted his paper to the same journal on February 10, 1953. Wilde's paper was published on July 10, 1953. Wilde used voltages up to only 600 V on a binary mixture of carbon dioxide (CO<sub>2</sub>) and water in a flow system. He observed only small amounts of carbon dioxide reduction to carbon monoxide, and no other significant reduction products or newly formed carbon compounds.
K. A. Wilde submitted a paper to Science on December 15, 1952, before Miller submitted his paper to the same journal on February 10, 1953. Wilde's paper was published on July 10, 1953. Wilde used voltages up to only 600 V on a binary mixture of carbon dioxide (CO<sub>2</sub>) and water in a flow system. He observed only small amounts of carbon dioxide reduction to carbon monoxide, and no other significant reduction products or newly formed carbon compounds.
1952年12月15日K·A· 王尔德 K. A. Wilde向《科学》杂志提交了一篇论文，早于米勒于1953年2月10日向该杂志提交的论文<ref>{{cite journal |last=Wilde |first=Kenneth A. |authorlink= |first2=Bruno J. |last2=Zwolinski |first3=Ransom B. |last3=Parlin |date=July 1953 |title=The Reaction Occurring in CO<sub>2</sub>, <sub>2</sub>O Mixtures in a High-Frequency Electric Arc |journal=[[Science (journal)|Science]] |volume=118 |issue=3054 |pages=43–44 |id= |doi=10.1126/science.118.3054.43-a |pmid=13076175 |bibcode=1953Sci...118...43W |df= }}</ref> 。王尔德的论文发表于1953年7月10日。王尔德将只有600v的电压施加于在流动系统中由<font color="#ff8000"> 二氧化碳 carbon dioxide</font>(CO2)和水所形成的二元混合物。他观察到只有少量的二氧化碳减少为<font color="#ff8000">一氧化碳 carbon dioxide </font>，没有其他重要的还原产物或新形成的碳化合物。
1952年12月15日K·A· 王尔德 K. A. Wilde向《科学》杂志提交了一篇论文，早于Miller于1953年2月10日向该杂志提交的论文<ref>{{cite journal |last=Wilde |first=Kenneth A. |authorlink= |first2=Bruno J. |last2=Zwolinski |first3=Ransom B. |last3=Parlin |date=July 1953 |title=The Reaction Occurring in CO<sub>2</sub>, <sub>2</sub>O Mixtures in a High-Frequency Electric Arc |journal=[[Science (journal)|Science]] |volume=118 |issue=3054 |pages=43–44 |id= |doi=10.1126/science.118.3054.43-a |pmid=13076175 |bibcode=1953Sci...118...43W |df= }}</ref> .Wilde的论文发表于1953年7月10日。Wilde将只有600v的电压施加于在流动系统中由<font color="#ff8000"> 二氧化碳 carbon dioxide</font>(CO2)和水所形成的二元混合物。他观察到只有少量的二氧化碳减少为<font color="#ff8000">一氧化碳 carbon dioxide </font>，没有其他重要的还原产物或新形成的碳化合物。
More recent experiments by chemists Jeffrey Bada, one of Miller's graduate students, and Jim Cleaves at Scripps Institution of Oceanography of the University of California, San Diego were similar to those performed by Miller. However, Bada noted that in current models of early Earth conditions, carbon dioxide and nitrogen (N<sub>2</sub>) create nitrites, which destroy amino acids as fast as they form. <!--However, the early Earth may have had significant amounts of iron and carbonate minerals able to neutralize the effects of the nitrites. --> <!-- Please find a scientific paper that makes this statement before removing the tag -- and then the remark may be visible again --> When Bada performed the Miller-type experiment with the addition of iron and carbonate minerals, the products were rich in amino acids. This suggests the origin of significant amounts of amino acids may have occurred on Earth even with an atmosphere containing carbon dioxide and nitrogen.
More recent experiments by chemists Jeffrey Bada, one of Miller's graduate students, and Jim Cleaves at Scripps Institution of Oceanography of the University of California, San Diego were similar to those performed by Miller. However, Bada noted that in current models of early Earth conditions, carbon dioxide and nitrogen (N<sub>2</sub>) create nitrites, which destroy amino acids as fast as they form. <!--However, the early Earth may have had significant amounts of iron and carbonate minerals able to neutralize the effects of the nitrites. --> <!-- Please find a scientific paper that makes this statement before removing the tag -- and then the remark may be visible again --> When Bada performed the Miller-type experiment with the addition of iron and carbonate minerals, the products were rich in amino acids. This suggests the origin of significant amounts of amino acids may have occurred on Earth even with an atmosphere containing carbon dioxide and nitrogen.
米勒的研究生之一、化学家Jeffery Bade和加州大学圣地亚哥斯克里普斯海洋学研究所的吉姆·克里夫斯 Jim Cleaves最近的实验与Miller的实验相似。然而，Bade指出，在目前的地球早期条件模型中，二氧化碳和<font color="#ff8000"> 氮 nitrogen</font>（N2）会产生<font color="#ff8000"> 亚硝酸盐 nitrites</font>，这会立即破坏氨基酸。<!--然而，早期地球可能有大量的铁和碳酸盐矿物能够中和亚硝酸盐的影响。--> <!--在去掉标签之前，请先找到一篇科学论文来说明这一点——然后这句话可能会再次显现出来-->当Bade进行Miller式实验时，他添加了铁和碳酸盐矿物，制作出的产品富含氨基酸。这表明，即使是含有二氧化碳和氮气的大气中，也可能成为大量氨基酸的起源之处<ref name=Fox>{{Cite news |last=Fox |first=Douglas |date=2007-03-28 |title=Primordial Soup's On: Scientists Repeat Evolution's Most Famous Experiment |periodical=Scientific American |series=History of Science |publisher=Scientific American Inc. |url=http://www.sciam.com/article.cfm?id=primordial-soup-urey-miller-evolution-experiment-repeated |accessdate=2008-07-09 }}<br>{{Cite journal | last1 = Cleaves | first1 = H. J. | last2 = Chalmers | first2 = J. H. | last3 = Lazcano | first3 = A. | last4 = Miller | first4 = S. L. | last5 = Bada | first5 = J. L. | title = A Reassessment of Prebiotic Organic Synthesis in Neutral Planetary Atmospheres | doi = 10.1007/s11084-007-9120-3 | journal = Origins of Life and Evolution of Biospheres | volume = 38 | issue = 2 | pages = 105–115 | year = 2008 | pmid = 18204914| bibcode = 2008OLEB...38..105C |url=http://www.astro.ulg.ac.be/~mouchet/BIOC0701-1/Cleaves-etal-2008.pdf |url-status=dead |archive-url=https://web.archive.org/web/20131107134729/http://www.astro.ulg.ac.be/~mouchet/BIOC0701-1/Cleaves-etal-2008.pdf |archive-date=2013-11-07 }}</ref>
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Some evidence suggests that Earth's original atmosphere might have contained fewer of the reducing molecules than was thought at the time of the Miller–Urey experiment. There is abundant evidence of major volcanic eruptions 4 billion years ago, which would have released carbon dioxide, nitrogen, hydrogen sulfide (H<sub>2</sub>S), and sulfur dioxide (SO<sub>2</sub>) into the atmosphere. Experiments using these gases in addition to the ones in the original Miller–Urey experiment have produced more diverse molecules. The experiment created a mixture that was racemic (containing both L and D enantiomers) and experiments since have shown that "in the lab the two versions are equally likely to appear"; however, in nature, L amino acids dominate. Later experiments have confirmed disproportionate amounts of L or D oriented enantiomers are possible.
Some evidence suggests that Earth's original atmosphere might have contained fewer of the reducing molecules than was thought at the time of the Miller–Urey experiment. There is abundant evidence of major volcanic eruptions 4 billion years ago, which would have released carbon dioxide, nitrogen, hydrogen sulfide (H<sub>2</sub>S), and sulfur dioxide (SO<sub>2</sub>) into the atmosphere. Experiments using these gases in addition to the ones in the original Miller–Urey experiment have produced more diverse molecules. The experiment created a mixture that was racemic (containing both L and D enantiomers) and experiments since have shown that "in the lab the two versions are equally likely to appear"; however, in nature, L amino acids dominate. Later experiments have confirmed disproportionate amounts of L or D oriented enantiomers are possible.
一些证据表明，地球原始大气层中还原分子的含量可能比Miller-Urey实验时所认为的要少。有大量的证据表明，40亿年前的大型火山爆发会向大气中释放二氧化碳、氮、<font color="#ff8000"> 硫化氢 hydrogen sulfide</font>(H2S)和<font color="#ff8000">二氧化硫 sulfur dioxide </font>(SO2) <ref name=Green>{{Cite journal|last=Green|first=Jack|title=Academic Aspects of Lunar Water Resources and Their Relevance to Lunar Protolife|journal=International Journal of Molecular Sciences|year=2011|volume=12|issue=9|pages=6051–6076|doi=10.3390/ijms12096051|pmid=22016644|pmc=3189768|ref=harv}}</ref>。除了最初的Miller-Urey实验中使用的气体之外，进一步使用这些气体的实验产生了更多样化的分子。该实验创造了一种外消旋体(包含L和D对映异构体)的混合物。此后的实验表明，“在实验室中，这两种化合物出现的可能性相等” <ref name="NS">{{Cite news |date=2006-06-02 |title=Right-handed amino acids were left behind |periodical=[[New Scientist]] |publisher=Reed Business Information Ltd |issue=2554 |pages=18 |url=https://www.newscientist.com/channel/life/mg19025545.200-righthanded-amino-acids-were-left-behind.html |accessdate=2008-07-09 |url-status=live |archiveurl=https://web.archive.org/web/20081024211531/http://www.newscientist.com/channel/life/mg19025545.200-righthanded-amino-acids-were-left-behind.html |archivedate=2008-10-24 }}</ref> ; 然而，在自然界中，L氨基酸占主导地位。后来的实验证实了不成比例的L或D取向对映异构体是可能的<ref>{{cite journal |last=Kojo |first=Shosuke |first2=Hiromi |last2=Uchino |first3=Mayu |last3=Yoshimura |first4=Kyoko |last4=Tanaka |date=October 2004 |title=Racemic D,L-asparagine causes enantiomeric excess of other coexisting racemic D,L-amino acids during recrystallization: a hypothesis accounting for the origin of L-amino acids in the biosphere |journal=Chemical Communications |volume= |issue=19 |pages=2146–2147 |pmid=15467844 |doi=10.1039/b409941a}}</ref>
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Originally it was thought that the primitive secondary atmosphere contained mostly ammonia and methane. However, it is likely that most of the atmospheric carbon was CO<sub>2</sub> with perhaps some CO and the nitrogen mostly N<sub>2</sub>. In practice gas mixtures containing CO, CO<sub>2</sub>, N<sub>2</sub>, etc. give much the same products as those containing CH<sub>4</sub> and NH<sub>3</sub> so long as there is no O<sub>2</sub>. The hydrogen atoms come mostly from water vapor. In fact, in order to generate aromatic amino acids under primitive earth conditions it is necessary to use less hydrogen-rich gaseous mixtures. Most of the natural amino acids, hydroxyacids, purines, pyrimidines, and sugars have been made in variants of the Miller experiment.
Originally it was thought that the primitive secondary atmosphere contained mostly ammonia and methane. However, it is likely that most of the atmospheric carbon was CO<sub>2</sub> with perhaps some CO and the nitrogen mostly N<sub>2</sub>. In practice gas mixtures containing CO, CO<sub>2</sub>, N<sub>2</sub>, etc. give much the same products as those containing CH<sub>4</sub> and NH<sub>3</sub> so long as there is no O<sub>2</sub>. The hydrogen atoms come mostly from water vapor. In fact, in order to generate aromatic amino acids under primitive earth conditions it is necessary to use less hydrogen-rich gaseous mixtures. Most of the natural amino acids, hydroxyacids, purines, pyrimidines, and sugars have been made in variants of the Miller experiment.
起初人们认为，原始次生大气主要含有氨和甲烷。然而，大气中的大部分碳可能是二氧化碳 ，一些一氧化碳和氮——大部分是氮气。在实际应用中，含有一氧化碳，二氧化碳和氮气等的混合气体在没有<font color="#ff8000">氧气 oxygen </font>的条件下可以给出与含甲烷和氨气的混合气体制造出的产品相一致的产物。氢原子主要来自水蒸气。事实上，为了在原始地球条件下生成<font color="#ff8000">芳香族氨基酸 aromatic </font>，必须使用较少的富氢气体混合物。大多数天然氨基酸、<font color="#ff8000">羟基酸 hydroxyacids</font><font color="#ff8000">嘌呤 purines </font>、<font color="#ff8000">嘧啶 pyrimidines </font>和糖都在米勒实验的变体中生成<ref name=bada2013/><ref>{{cite journal|last1=Ruiz-Mirazo|first1=Kepa|last2=Briones|first2=Carlos|last3=de la Escosura|first3=Andrés|title=Prebiotic Systems Chemistry: New Perspectives for the Origins of Life|journal=Chemical Reviews|year=2014|volume=114|issue=1|pages=285–366|doi=10.1021/cr2004844|pmid=24171674}}</ref>
起初人们认为，原始次生大气主要含有氨和甲烷。然而，大气中的大部分碳可能是二氧化碳 ，一些一氧化碳和氮——大部分是氮气。在实际应用中，含有一氧化碳，二氧化碳和氮气等的混合气体在没有<font color="#ff8000">氧气 oxygen </font>的条件下可以给出与含甲烷和氨气的混合气体制造出的产品相一致的产物。氢原子主要来自水蒸气。事实上，为了在原始地球条件下生成<font color="#ff8000">芳香族氨基酸 aromatic </font>，必须使用较少的富氢气体混合物。大多数天然氨基酸、<font color="#ff8000">羟基酸 hydroxyacids</font><font color="#ff8000">嘌呤 purines </font>、<font color="#ff8000">嘧啶 pyrimidines </font>和糖都在Miller实验的变体中生成<ref name=bada2013/><ref>{{cite journal|last1=Ruiz-Mirazo|first1=Kepa|last2=Briones|first2=Carlos|last3=de la Escosura|first3=Andrés|title=Prebiotic Systems Chemistry: New Perspectives for the Origins of Life|journal=Chemical Reviews|year=2014|volume=114|issue=1|pages=285–366|doi=10.1021/cr2004844|pmid=24171674}}</ref>
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Conditions similar to those of the Miller–Urey experiments are present in other regions of the solar system, often substituting ultraviolet light for lightning as the energy source for chemical reactions. The Murchison meteorite that fell near Murchison, Victoria, Australia in 1969 was found to contain over 90 different amino acids, nineteen of which are found in Earth life. Comets and other icy outer-solar-system bodies are thought to contain large amounts of complex carbon compounds (such as tholins) formed by these processes, darkening surfaces of these bodies. The early Earth was bombarded heavily by comets, possibly providing a large supply of complex organic molecules along with the water and other volatiles they contributed. This has been used to infer an origin of life outside of Earth: the panspermia hypothesis.
Conditions similar to those of the Miller–Urey experiments are present in other regions of the solar system, often substituting ultraviolet light for lightning as the energy source for chemical reactions. The Murchison meteorite that fell near Murchison, Victoria, Australia in 1969 was found to contain over 90 different amino acids, nineteen of which are found in Earth life. Comets and other icy outer-solar-system bodies are thought to contain large amounts of complex carbon compounds (such as tholins) formed by these processes, darkening surfaces of these bodies. The early Earth was bombarded heavily by comets, possibly providing a large supply of complex organic molecules along with the water and other volatiles they contributed. This has been used to infer an origin of life outside of Earth: the panspermia hypothesis.
类似Miller-Urey实验条件的环境在太阳系的其他区域也存在——不过通常以紫外线代替闪电作为化学反应的能源<ref>{{cite journal|last1=Nunn|first1=JF|title=Evolution of the atmosphere|journal=Proceedings of the Geologists' Association. Geologists' Association|year=1998|volume=109|issue=1|pages=1–13|pmid=11543127|doi=10.1016/s0016-7878(98)80001-1}}</ref><ref>{{cite journal|last1=Raulin|first1=F|last2=Bossard|first2=A|title=Organic syntheses in gas phase and chemical evolution in planetary atmospheres.|journal=Advances in Space Research|year=1984|volume=4|issue=12|pages=75–82|pmid=11537798|doi=10.1016/0273-1177(84)90547-7|bibcode=1984AdSpR...4...75R}}</ref><ref>{{cite journal|last1=Raulin|first1=François|last2=Brassé|first2=Coralie|last3=Poch|first3=Olivier|last4=Coll|first4=Patrice|title=Prebiotic-like chemistry on Titan|journal= Chemical Society Reviews|year=2012|volume=41|issue=16|pages=5380–93|doi=10.1039/c2cs35014a|pmid=22481630}}</ref>。1969年落在澳大利亚维多利亚州默奇森河附近的默奇森陨石被发现含有超过90种不同的氨基酸，其中十九种存在于地球生命中。彗星和其他太阳系外围的冰冷天体被认为含有大量复杂的碳化合物(例如<font color="#ff8000">塞林 tholins </font>) ，在天体的暗化表面经由这些步骤形成<ref>{{cite journal |vauthors=Thompson WR, Murray BG, Khare BN, Sagan C |title=Coloration and darkening of methane clathrate and other ices by charged particle irradiation: applications to the outer solar system |journal=Journal of Geophysical Research |volume=92 |issue=A13 |pages=14933–47 |date=December 1987 |pmid=11542127 |doi=10.1029/JA092iA13p14933 |bibcode=1987JGR....9214933T|title-link=methane clathrate }}</ref>。早期的地球遭受了严重的彗星撞击，产生了大量复杂的有机分子以及水和其他挥发物<ref>{{cite journal|last=PIERAZZO|first=E.|author2=CHYBA C.F.|title=Amino acid survival in large cometary impacts|journal=Meteoritics & Planetary Science|year=2010|volume=34|issue=6|pages=909–918|doi=10.1111/j.1945-5100.1999.tb01409.x|bibcode=1999M&PS...34..909P}}</ref>。这被用来推断地球以外生命的起源: <font color="#ff8000">胚种论 the panspermia hypothesis
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Jeffrey Bada, himself Miller's student, inherited the original equipment from the experiment when Miller died in 2007. Based on sealed vials from the original experiment, scientists have been able to show that although successful, Miller was never able to find out, with the equipment available to him, the full extent of the experiment's success. Later researchers have been able to isolate even more different amino acids, 25 altogether. Bada has estimated that more accurate measurements could easily bring out 30 or 40 more amino acids in very low concentrations, but the researchers have since discontinued the testing. Miller's experiment was therefore a remarkable success at synthesizing complex organic molecules from simpler chemicals, considering that all known life uses just 20 different amino acids.
Jeffrey Bada, himself Miller's student, inherited the original equipment from the experiment when Miller died in 2007. Based on sealed vials from the original experiment, scientists have been able to show that although successful, Miller was never able to find out, with the equipment available to him, the full extent of the experiment's success. Later researchers have been able to isolate even more different amino acids, 25 altogether. Bada has estimated that more accurate measurements could easily bring out 30 or 40 more amino acids in very low concentrations, but the researchers have since discontinued the testing. Miller's experiment was therefore a remarkable success at synthesizing complex organic molecules from simpler chemicals, considering that all known life uses just 20 different amino acids.
Jeffery Bade是Miller的学生，他在2007年Miller去世时继承了这项实验的原始设备。根据最初实验中的密封小瓶，科学家们已经能够证明，虽然米勒成功了，但在现有设备条件下，Miller始终无法彻底的完成实验。后来的研究人员已经能够分离出更多不同的氨基酸，总共25种。Bade估测，在非常低的浓度下可以进行更精确地测量，从而提取出30或40种氨基酸，但是研究人员已经停止了这项测试。考虑到所有已知生命只使用20种不同的氨基酸，Miller的实验已经在从较简单的化学物质合成复杂有机分子方面取得了显著成功<ref name="BBC">{{cite web |website=BBC Four |url=http://www.bbc.co.uk/programmes/b00mbvfh |title=The Spark of Life |url-status=live |archive-url=https://web.archive.org/web/20101113011054/http://www.bbc.co.uk/programmes/b00mbvfh |archive-date=2010-11-13 |postscript=. TV Documentary. |date=26 August 2009}}</ref>
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In 2008, a group of scientists examined 11 vials left over from Miller's experiments of the early 1950s. In addition to the classic experiment, reminiscent of [[Charles Darwin]]'s envisioned "warm little pond", Miller had also performed more experiments, including one with conditions similar to those of [[volcano|volcanic]] eruptions. This experiment had a nozzle spraying a jet of steam at the spark discharge. By using [[high-performance liquid chromatography]] and [[mass spectrometry]], the group found more organic molecules than Miller had. They found that the volcano-like experiment had produced the most organic molecules, 22 amino acids, 5 [[amine]]s and many [[hydroxylate]]d molecules, which could have been formed by [[hydroxyl radical]]s produced by the electrified steam. The group suggested that volcanic island systems became rich in organic molecules in this way, and that the presence of [[carbonyl sulfide]] there could have helped these molecules form [[peptide]]s.<ref name=Johnson2008>{{cite journal |vauthors=Johnson AP, Cleaves HJ, Dworkin JP, Glavin DP, Lazcano A, Bada JL |title=The Miller volcanic spark discharge experiment |journal=Science |volume=322 |issue=5900 |pages=404 |date=October 2008 |pmid=18927386 |doi=10.1126/science.1161527|bibcode = 2008Sci...322..404J }}</ref><ref>{{cite web | title='Lost' Miller–Urey Experiment Created More Of Life's Building Blocks | date=October 17, 2008 | website=Science Daily | url=https://www.sciencedaily.com/releases/2008/10/081016141411.htm | accessdate=2008-10-18 | url-status=live | archiveurl=https://web.archive.org/web/20081019111114/http://www.sciencedaily.com/releases/2008/10/081016141411.htm | archivedate=October 19, 2008 }}</ref>
In 2008, a group of scientists examined 11 vials left over from Miller's experiments of the early 1950s. In addition to the classic experiment, reminiscent of [[Charles Darwin]]'s envisioned "warm little pond", Miller had also performed more experiments, including one with conditions similar to those of [[volcano|volcanic]] eruptions. This experiment had a nozzle spraying a jet of steam at the spark discharge. By using [[high-performance liquid chromatography]] and [[mass spectrometry]], the group found more organic molecules than Miller had. They found that the volcano-like experiment had produced the most organic molecules, 22 amino acids, 5 [[amine]]s and many [[hydroxylate]]d molecules, which could have been formed by [[hydroxyl radical]]s produced by the electrified steam. The group suggested that volcanic island systems became rich in organic molecules in this way, and that the presence of [[carbonyl sulfide]] there could have helped these molecules form [[peptide]]s.<ref name=Johnson2008>{{cite journal |vauthors=Johnson AP, Cleaves HJ, Dworkin JP, Glavin DP, Lazcano A, Bada JL |title=The Miller volcanic spark discharge experiment |journal=Science |volume=322 |issue=5900 |pages=404 |date=October 2008 |pmid=18927386 |doi=10.1126/science.1161527|bibcode = 2008Sci...322..404J }}</ref><ref>{{cite web | title='Lost' Miller–Urey Experiment Created More Of Life's Building Blocks | date=October 17, 2008 | website=Science Daily | url=https://www.sciencedaily.com/releases/2008/10/081016141411.htm | accessdate=2008-10-18 | url-status=live | archiveurl=https://web.archive.org/web/20081019111114/http://www.sciencedaily.com/releases/2008/10/081016141411.htm | archivedate=October 19, 2008 }}</ref>
In 2008, a group of scientists examined 11 vials left over from Miller's experiments of the early 1950s. In addition to the classic experiment, reminiscent of Charles Darwin's envisioned "warm little pond", Miller had also performed more experiments, including one with conditions similar to those of volcanic eruptions. This experiment had a nozzle spraying a jet of steam at the spark discharge. By using high-performance liquid chromatography and mass spectrometry, the group found more organic molecules than Miller had. They found that the volcano-like experiment had produced the most organic molecules, 22 amino acids, 5 amines and many hydroxylated molecules, which could have been formed by hydroxyl radicals produced by the electrified steam. The group suggested that volcanic island systems became rich in organic molecules in this way, and that the presence of carbonyl sulfide there could have helped these molecules form peptides.
In 2008, a group of scientists examined 11 vials left over from Miller's experiments of the early 1950s. In addition to the classic experiment, reminiscent of Charles Darwin's envisioned "warm little pond", Miller had also performed more experiments, including one with conditions similar to those of volcanic eruptions. This experiment had a nozzle spraying a jet of steam at the spark discharge. By using high-performance liquid chromatography and mass spectrometry, the group found more organic molecules than Miller had. They found that the volcano-like experiment had produced the most organic molecules, 22 amino acids, 5 amines and many hydroxylated molecules, which could have been formed by hydroxyl radicals produced by the electrified steam. The group suggested that volcanic island systems became rich in organic molecules in this way, and that the presence of carbonyl sulfide there could have helped these molecules form peptides.
2008年，一组科学家检查了Miller20世纪50年代早期实验中遗留下来的11个小瓶。除了这个经典实验外——让人想起查尔斯·达尔文 Charles Darwin设想的“温暖的小池塘”，米勒还进行了更多的实验，其中一个实验的条件与火山爆发时相似。这个实验有一个喷嘴在火花放电处喷射蒸汽。通过使用<font color="#ff8000">高效液相色谱 high-performance liquid chromatography</font>和<font color="#ff8000"> 质谱 mass spectrometry </font>，研究小组比Miller发现了更多的有机分子。他们发现，类似火山的实验产生了最多的有机分子，22个氨基酸，5个胺和许多羟基化分子，这些分子可能是由通电蒸汽产生的羟基自由基形成的。研究小组认为，火山岛系统因这种方式而富含有机分子，而羰基硫化物的存在可能有助于这些分子形成<font color="#ff8000">肽 peptides</font>。<ref name=Johnson2008>{{cite journal |vauthors=Johnson AP, Cleaves HJ, Dworkin JP, Glavin DP, Lazcano A, Bada JL |title=The Miller volcanic spark discharge experiment |journal=Science |volume=322 |issue=5900 |pages=404 |date=October 2008 |pmid=18927386 |doi=10.1126/science.1161527|bibcode = 2008Sci...322..404J }}</ref><ref>{{cite web | title='Lost' Miller–Urey Experiment Created More Of Life's Building Blocks | date=October 17, 2008 | website=Science Daily | url=https://www.sciencedaily.com/releases/2008/10/081016141411.htm | accessdate=2008-10-18 | url-status=live | archiveurl=https://web.archive.org/web/20081019111114/http://www.sciencedaily.com/releases/2008/10/081016141411.htm | archivedate=October 19, 2008 }}</ref>
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The main problem of theories based around amino acids is the difficulty in obtaining spontaneous formation of peptides. Since John Desmond Bernal's suggestion that clay surfaces could have played a role in abiogenesis, scientific efforts have been dedicated to investigating clay-mediated peptide bond formation, with limited success. Peptides formed remained over-protected and shown no evidence of inheritance or metabolism. In December 2017 a theoretical model developed by Erastova and collaborators suggested that peptides could form at the interlayers of layered double hydroxides such as green rust in early earth conditions. According to the model, drying of the intercalated layered material should provide energy and co-alignment required for peptide bond formation in a ribosome-like fashion, while re-wetting should allow mobilising the newly formed peptides and repopulate the interlayer with new amino acids. This mechanism is expected to lead to the formation of 12+ amino acid-long peptides within 15-20 washes. Researches also observed slightly different adsorption preferences for different amino acids, and postulated that, if coupled to a diluted solution of mixed amino acids, such preferences could lead to sequencing.
The main problem of theories based around amino acids is the difficulty in obtaining spontaneous formation of peptides. Since John Desmond Bernal's suggestion that clay surfaces could have played a role in abiogenesis, scientific efforts have been dedicated to investigating clay-mediated peptide bond formation, with limited success. Peptides formed remained over-protected and shown no evidence of inheritance or metabolism. In December 2017 a theoretical model developed by Erastova and collaborators suggested that peptides could form at the interlayers of layered double hydroxides such as green rust in early earth conditions. According to the model, drying of the intercalated layered material should provide energy and co-alignment required for peptide bond formation in a ribosome-like fashion, while re-wetting should allow mobilising the newly formed peptides and repopulate the interlayer with new amino acids. This mechanism is expected to lead to the formation of 12+ amino acid-long peptides within 15-20 washes. Researches also observed slightly different adsorption preferences for different amino acids, and postulated that, if coupled to a diluted solution of mixed amino acids, such preferences could lead to sequencing.
以氨基酸为基础的理论的主要问题是很难获得自发形成的肽。自从约翰·德斯蒙德·伯纳尔John Desmond Bernal提出粘土表面可能在自然发生中起作用这一构想以来<ref name=Bernal1949>{{cite journal |vauthors=Bernal JD |title=The physical basis of life |journal=Proc. Phys. Soc. A | issue=9 |volume=62 |pages=537–558 |date=1949|doi=10.1088/0370-1298/62/9/301 |bibcode=1949PPSA...62..537B }}</ref>，科学家就致力于研究粘土介导的肽键的形成，但成效有限。形成的肽保护过度，没有表现出遗传或新陈代谢的特征。2017年12月，伊拉斯托瓦Erastova和他的合作者<ref name="RT-2018">{{cite news | publisher=RT | url=https://www.rt.com/news/416581-scientists-unlock-life-puzzle-protein/ | title='How did life form from rocks?' Protein puzzle reveals secrets of Earth's evolution | date=January 2017}}</ref><ref name="Erastova2017">{{cite journal |vauthors=Erastova V, Degiacomi MT, Fraser D, Greenwell HC |title=Mineral surface chemistry control for origin of prebiotic peptides |journal=Nature Communications |volume=8 |issue=1 |pages=2033 |date=December 2017|pmid=29229963 |pmc=5725419 |doi=10.1038/s41467-017-02248-y |bibcode=2017NatCo...8.2033E }}</ref>开发的一个理论模型表明，在早期的地球条件下，肽可以在层状双氢氧化物的中间层形成，例如绿锈。根据该模型，干燥的插层材料应为肽键的形成提供能量和，并以核糖体样的方式形成肽键所需的共排列，而再湿润应允许活化新形成的肽以及用新的氨基酸重新填充中间层。这一机制有望在15-20次的洗涤中形成12 + 氨基酸长肽。研究人员还观察到对不同氨基酸的吸附偏好略有不同，并假设，如果与混合氨基酸的稀释溶液相结合，这种偏好可能会导致排序。
以氨基酸为基础的理论的主要问题是很难获得自发形成的肽。自从约翰·德斯蒙德·伯纳尔John Desmond Bernal提出粘土表面可能在自然发生中起作用这一构想以来<ref name=Bernal1949>{{cite journal |vauthors=Bernal JD |title=The physical basis of life |journal=Proc. Phys. Soc. A | issue=9 |volume=62 |pages=537–558 |date=1949|doi=10.1088/0370-1298/62/9/301 |bibcode=1949PPSA...62..537B }}</ref>，科学家就致力于研究粘土介导的肽键的形成，但成效有限。形成的肽保护过度，没有表现出遗传或新陈代谢的特征。2017年12月，伊拉斯托瓦 Erastova和他的合作者<ref name="RT-2018">{{cite news | publisher=RT | url=https://www.rt.com/news/416581-scientists-unlock-life-puzzle-protein/ | title='How did life form from rocks?' Protein puzzle reveals secrets of Earth's evolution | date=January 2017}}</ref><ref name="Erastova2017">{{cite journal |vauthors=Erastova V, Degiacomi MT, Fraser D, Greenwell HC |title=Mineral surface chemistry control for origin of prebiotic peptides |journal=Nature Communications |volume=8 |issue=1 |pages=2033 |date=December 2017|pmid=29229963 |pmc=5725419 |doi=10.1038/s41467-017-02248-y |bibcode=2017NatCo...8.2033E }}</ref>开发的一个理论模型表明，在早期的地球条件下，肽可以在层状双氢氧化物的中间层形成，例如绿锈。根据该模型，干燥的插层材料应为肽键的形成提供能量和，并以核糖体样的方式形成肽键所需的共排列，而再湿润应允许活化新形成的肽以及用新的氨基酸重新填充中间层。这一机制有望在15-20次的洗涤中形成12 + 氨基酸长肽。研究人员还观察到对不同氨基酸的吸附偏好略有不同，并假设，如果与混合氨基酸的稀释溶液相结合，这种偏好可能会导致排序。
In October 2018, researchers at [[McMaster University]] on behalf of the [[Origins Institute]] announced the development of a new technology, called a ''[[Planet Simulator]]'', to help study the [[origin of life]] on planet [[Earth]] and beyond.<ref name="BW-20181004">{{cite news |last=Balch |first=Erica |title=Ground-breaking lab poised to unlock the mystery of the origins of life on Earth and beyond |url=https://brighterworld.mcmaster.ca/articles/ground-breaking-lab-poised-to-unlock-the-mystery-of-the-origins-of-life-on-earth-and-beyond/ |date=4 October 2018 |work=[[McMaster University]] |accessdate=4 October 2018 }}</ref><ref name="EA-20181004">{{cite news |author=Staff |title=Ground-breaking lab poised to unlock the mystery of the origins of life |url=https://www.eurekalert.org/pub_releases/2018-10/mu-glp100418.php |date=4 October 2018 |work=[[EurekAlert!]] |accessdate=14 October 2018 }}</ref><ref name="IVG-2018">{{cite web |author=Staff |title=Planet Simulator |url=https://www.intravisiongroup.com/planet-simulator |date=2018 |work=IntraVisionGroup.com |accessdate=14 October 2018 }}</ref><ref name="ES-209181014">{{cite web |last=Anderson |first=Paul Scott |title=New technology may help solve mystery of life's origins - How did life on Earth begin? A new technology, called Planet Simulator, might finally help solve the mystery. |url=http://earthsky.org/space/new-technology-solve-mystery-of-lifes-origins |date=14 October 2018 |work=[[EarthSky]] |accessdate=14 October 2018 }}</ref>
In October 2018, researchers at [[McMaster University]] on behalf of the [[Origins Institute]] announced the development of a new technology, called a ''[[Planet Simulator]]'', to help study the [[origin of life]] on planet [[Earth]] and beyond.<ref name="BW-20181004">{{cite news |last=Balch |first=Erica |title=Ground-breaking lab poised to unlock the mystery of the origins of life on Earth and beyond |url=https://brighterworld.mcmaster.ca/articles/ground-breaking-lab-poised-to-unlock-the-mystery-of-the-origins-of-life-on-earth-and-beyond/ |date=4 October 2018 |work=[[McMaster University]] |accessdate=4 October 2018 }}</ref><ref name="EA-20181004">{{cite news |author=Staff |title=Ground-breaking lab poised to unlock the mystery of the origins of life |url=https://www.eurekalert.org/pub_releases/2018-10/mu-glp100418.php |date=4 October 2018 |work=[[EurekAlert!]] |accessdate=14 October 2018 }}</ref><ref name="IVG-2018">{{cite web |author=Staff |title=Planet Simulator |url=https://www.intravisiongroup.com/planet-simulator |date=2018 |work=IntraVisionGroup.com |accessdate=14 October 2018 }}</ref><ref name="ES-209181014">{{cite web |last=Anderson |first=Paul Scott |title=New technology may help solve mystery of life's origins - How did life on Earth begin? A new technology, called Planet Simulator, might finally help solve the mystery. |url=http://earthsky.org/space/new-technology-solve-mystery-of-lifes-origins |date=14 October 2018 |work=[[EarthSky]] |accessdate=14 October 2018 }}</ref>
Below is a table of amino acids produced and identified in the "classic" 1952 experiment, as published by Miller in 1953, and the 2010 re-analysis of vials from the H<sub>2</sub>S-rich spark discharge experiment.
Below is a table of amino acids produced and identified in the "classic" 1952 experiment, as published by Miller in 1953, and the 2010 re-analysis of vials from the H<sub>2</sub>S-rich spark discharge experiment.
下面是依据1953年Miller发表的论文给出的1952年“经典”实验中产生并经过鉴定的氨基酸表<ref>{{cite web|last1=Myers|first1=P. Z.|title=Old scientists never clean out their refrigerators|url=http://scienceblogs.com/pharyngula/2008/10/old_scientists_never_clean_out.php|website=Pharyngula|accessdate=7 April 2016|archiveurl=https://web.archive.org/web/20081017231050/http://scienceblogs.com/pharyngula/2008/10/old_scientists_never_clean_out.php|archivedate=October 17, 2008|date=October 16, 2008}}</ref>，以及2010年对H2S高密度火花放电实验中小瓶的重新分析<ref>{{cite journal|title=Primordial synthesis of amines and amino acids in a 1958 Miller H2S-rich spark discharge experiment|journal=Proceedings of the National Academy of Sciences|date=February 14, 2011|volume=108|issue=14|doi=10.1073/pnas.1019191108|pmid=21422282|pmc=3078417|pages=5526–31|last1=Parker|first1=ET|last2=Cleaves|first2=HJ|last3=Dworkin|first3=JP|display-authors=etal |bibcode=2011PNAS..108.5526P|df=}}</ref>
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