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实验用水(H2O)、'''甲烷 methane'''(CH4)、'''氨 ammonia'''(NH3)和'''氢 hydrogen '''(H 2)。所有的化学物质都被密封在一个5升的无菌玻璃瓶里，这个玻璃瓶连接着一个500毫升的半满水的烧瓶。将小烧瓶中的水加热以诱导蒸发，使水蒸气进入大烧瓶。在电极之间连续地点燃电火花，在水蒸气和气体混合物中模拟闪电。然后再次冷却模拟的大气，使水凝结并滴入装置底部的U形曲管中。

实验用水(H₂O)、甲烷(CH4)、氨(NH3)和氢(H2)。所有的化学物质都被密封在一个5升的无菌玻璃瓶里，这个玻璃瓶连接着一个500毫升的半满水的烧瓶。将小烧瓶中的水加热以诱导蒸发，使水蒸气进入大烧瓶。在电极之间连续地点燃电火花，在水蒸气和气体混合物中模拟闪电。然后再次冷却模拟的大气，使水凝结并滴入装置底部的U形曲管中。

一天之后，在管内收集到的溶液变成了粉红色，而在连续操作一周之后，溶液变成了深红并且混浊的液体<ref name=miller1953/> The boiling flask was then removed, and mercuric chloride was added to prevent microbial contamination. The reaction was stopped by adding barium hydroxide and sulfuric acid, and evaporated to remove impurities. Using [[paper chromatography]], Miller identified five amino acids present in the solution: [[glycine]], [[alanine|α-alanine]] and [[beta-Alanine|β-alanine]] were positively identified, while [[aspartic acid]] and [[alpha-Aminobutyric acid|α-aminobutyric acid]] (AABA) were less certain, due to the spots being faint.<ref name=miller1953/>。

一天之后，在管内收集到的溶液变成了粉红色，而在连续操作一周之后，溶液变成了深红并且混浊的液体<ref name=miller1953/> 然后取出沸腾烧瓶，加入氯化汞以防止微生物污染。加入氢氧化钡和硫酸终止反应，蒸发除去杂质。使用纸色谱法，Miller 确定了溶液中存在的五种氨基酸：甘氨酸、α-丙氨酸和β-丙氨酸被阳性鉴定，而天冬氨酸和α-氨基丁酸(AABA) 不太确定，因为斑点是微弱的。<ref name=miller1953/>。

混合组分之间的一步反应可以生成'''氢化氢 hydrogen'''、'''甲醛 formaldehyde '''<ref>https://www.webcitation.org/query?url=http://www.geocities.com/capecanaveral/lab/2948/orgel.html&date=2009-10-25+16:53:26 Origin of Life on Earth by Leslie E. Orgel</ref><ref>{{Cite book |url=http://books.nap.edu/openbook.php?record_id=11860&page=85 |title=Read "Exploring Organic Environments in the Solar System" at NAP.edu |accessdate=2008-10-25 |url-status=live |archiveurl=https://web.archive.org/web/20090621053626/http://books.nap.edu/openbook.php?record_id=11860&page=85 |archivedate=2009-06-21 |doi=10.17226/11860 |year=2007 |isbn=978-0-309-10235-3 |last1=Council |first1=National Research |last2=Studies |first2=Division on Earth Life |last3=Technology |first3=Board on Chemical Sciences and |last4=Sciences |first4=Division on Engineering Physical |last5=Board |first5=Space Studies |last6=System |first6=Task Group on Organic Environments in the Solar }} Exploring Organic Environments in the Solar System (2007)</ref>和其他活性中间体('''乙炔 acetylene'''、'''氰乙炔 cyanoacetylene'''等) :{{Citation needed|date=June 2016}}

混合组分之间的一步反应可以生成'''氢化氢 hydrogen'''、'''甲醛 formaldehyde '''<ref>https://www.webcitation.org/query?url=http://www.geocities.com/capecanaveral/lab/2948/orgel.html&date=2009-10-25+16:53:26 Origin of Life on Earth by Leslie E. Orgel</ref><ref>{{Cite book |url=http://books.nap.edu/openbook.php?record_id=11860&page=85 |title=Read "Exploring Organic Environments in the Solar System" at NAP.edu |accessdate=2008-10-25 |url-status=live |archiveurl=https://web.archive.org/web/20090621053626/http://books.nap.edu/openbook.php?record_id=11860&page=85 |archivedate=2009-06-21 |doi=10.17226/11860 |year=2007 |isbn=978-0-309-10235-3 |last1=Council |first1=National Research |last2=Studies |first2=Division on Earth Life |last3=Technology |first3=Board on Chemical Sciences and |last4=Sciences |first4=Division on Engineering Physical |last5=Board |first5=Space Studies |last6=System |first6=Task Group on Organic Environments in the Solar }} Exploring Organic Environments in the Solar System (2007)</ref>和其他活性中间体(乙炔、氰乙炔等) :

==实验化学==

==实验化学==